Figure 6

XIAP protects MECs from apoptosis. FSK7 cells transiently expressing monomeric RFP or RFPXIAP were treated with staurosporine (STS) for 4 or 8 hours cells, then fixed and stained with DAPI. (A) Apoptosis was scored as the percentage of cells with apoptotic nuclei. (B) FSK7 cells transiently expressing monomeric RFP or RFPXIAP were serum starved in the presence of 10 μM Iressa for times shown and percentage apoptosis scored. Data shown represents the average of 3 independent experiments +/- S.E.M. (*** indicates p < 0.01). (C) Representative images of mRFP and RFPXIAP expressing cells (left panels and right panels, respectively) that were either left untreated (i), treated with STS for 4 hours (ii) or treated with Iressa for 6 hours (iii). Arrows and arrowheads indicate non-apoptotic and apoptotic nuclei, respectively. Note that the RFPXIAP-expressing drug treated cells are negative for active caspase 3 and have non-apoptotic nuclei. In contrast RFP-expressing cells are stained positive for active-caspase 3 and have condensed nuclei.