Figure 4

Spermatids from dmfrn^SH115flies show defects in the maturation of the mitochondrial derivatives. Transmission electron micrographs of ultra thin sections of testis from WT flies (A-C) and dmfrn^SH115flies (D-I). (A) Overview and (B) close-up of pre-individualization spermatids, showing the symmetric distribution of the spermatids within the cyst. The major mitochondrial derivative can easily be distinguished from the minor mitochondrial derivative by the accumulation of the electron dense stain. (C) Overview of individualized spermatids, which shows parachrystalline symmetry, a clearly visible major mitochondrial derivative and depletion of cytoplasm. (D) Overview and (E) close-up of dmfrn^SH115pre-individualization spermatids. No symmetry of the spermatids can be observed and accumulation of the paracrystalline structure to only a few major mitochondrial derivatives can be seen. (F and H) Overview and (G and I) close up of spermatids from dmfrn^SH115flies. Several axonemes are within the same spermatids and the accumulation of the paracrystalline structure within mitochondria is very heterogeneous.