Figure 3
Prestalk and prespore cell patterning in cpnA - slugs. Wild-type (A-C) and cpnA- (D-F) Dictyostelium cells transformed with the pspA-Gal (A, D) ecmAO-Gal (B, E), and ecmB-Gal (C, F) plasmids were plated on white filters in starvation buffer at 5 × 107 cells/mL and allowed to develop for 24 hours. At ~16 hours of development, slug structures were fixed and stained with X-Gal to reveal prestalk and prespore patterning. Images were taken with the Nikon SMZ1500 dissecting microscope equipped with a SPOT Flex colored camera using the SPOT digital imaging software (scale bar = 500 μm).