Figure 2
Time-lapse imaging revealed that cpnA - cells are delayed in aggregation and form larger mounds that develop into fewer, larger slugs. Wild-type (A-C, G-I) and cpnA- (D-F, J-L) Dictyostelium cells expressing GFP were plated on black filters in starvation buffer at 5 × 107 cells/mL and imaged every 10 minutes for 28 hours using a Leica dissecting fluorescence microscope at 20× magnification (scale bar = 1 mm). Hours:minutes in development are given in the lower right corner of each image. Arrow (K) points to stalk-like structure. Also see time-lapse movies in Additional Files 1 and 2.