Ablim1 lateral plate asymmetry is maintained in the absence of Nodal. Expression, as assessed by WISH with the 3'UTRa probe. In iv/iv mutants (a-c), (a) left-sided, (b) bilateral and (c) right sided expression was detected. (d) Left-sided Ablim1 expression in a NodalD600/D600 (D600/D600) embryo. (e) Normal, left-sided expression of Ablim1 in a Pitx2c-/- embryo. NodalΔnode/- (node/-) embryos show either loss of asymmetric Ablim1 expression (f) or left-sided Ablim1 expression (g). The posterior most extent of LPM expression is indicated by a closed arrowhead.