| UAS-Tc-bhsp-tGFP |
---|
 | #2 | #7 |
---|
Tc-hsp-GAL4Δ #1 | 3 h | 4 h |
Tc-hsp-GAL4Δ #2 | 3 h | 4 h |
Tc-hsp-GAL4-VP16 #2 | 4 h | 4 h |
Tc-hsp-GAL4-VP16 #3 | 4 h | 6 h |
- Response to GAL4Δ is slightly faster than to GAL4-VP16. Shown is the time when first fluorescence of tGFP was visible after heat shock. The driver lines Tc-hsp-GAL4Δ#1 and #2 as well as Tc-hsp-GAL4-VP16#2 and #3 were crossed against the responder lines UAS-Tc-bhsp-tGFP#2 and #7. Combinations containing driver lines based on GAL4Δ result in earlier responder gene expression (3.5 hours on average) than combinations containing GAL4-VP16-based lines (4.5 hours on average).