Figure 7

Myc-Fry-GFP moves rapidly in da neurons and bristles. A FRAP experiment on a single ppkGAL4/UAS-myc-fry-GFP da neuron is shown in A. One image is prior to bleaching. The next image is immediately after bleaching. The subsequent images were taken at 33.5 second intervals. Note that by 67 seconds substantial recovery has take place and it was essentially complete by 2 minutes. A FRAP experiment on an actinGAL4/UAS-myc-fry-GFP; fry¹/fry² bristle. The recovery in this experiment was quite rapid and was complete by 30 seconds. N-U, non-bleached upper position; B-U, bleached upper position; B-M, bleached middle position; B-L, bleached lower position; N-L, non-bleached lower position. Panel C shows the intensity of GFP at various times at several locations in the bristle shown in Panel B. 1-7 refer to 7 different time points. 1, pre-bleach; 2, 0 second (right after bleach); 3, 6.378 seconds; 4, 12.757 seconds; 5, 19.134 seconds; 6, 25.514 seconds; 7, 31.892 seconds.