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Table 1 Muc1IC2 lineage contribution to exocrine cells

From: Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas

     

Lineage+(EYFP or LacZ)

Experiment

Genotype

Pulse protocol

Chase period

Sample #

% of DAPI+

% of AMY+

% of CK19+

1

Muc1 IC2/+ ; Rosa26 EYFP/+

adult (P60)

10 mg TM

gavage

7 d

1

23.6

29.3

7.5

    

2

22

27.3

6.2

    

3

24.3

32.2

5.4

    

mean

23.3 ± 0.7

29.6 ± 1.4

6.4 ± 0.6

   

120 d

1

30

42.4

8.5

    

2

12.6

15

11.7

    

3

20.8

26.4

10.1

    

4

33

43.6

14.5

    

5

23.6

30.6

13.3

    

mean

24.0 ± 3.6

31.6 ± 5.3

11.6 ± 1.1

   

overall mean

 

23.7 ± 2.2

30.8 ± 3.2

9.6 ± 1.2

2

Muc1 IC2/+ ; Rosa26 LacZ/+

pups

(P0/P1)

10 mg TM

maternal gavage

21 d

1

16.6

22.9

9.9

    

2

11.7

15.5

3.6

    

3

3.3

4.1

1.8

    

4

6.2

7.4

1.9

    

5

5.1

5.9

1.5

    

mean

8.6 ± 2.4

11.2 ± 3.5

3.7 ± 1.6

   

120 d

1

14.1

21.9

5.5

    

2

21.4

30.9

6.2

    

mean

17.7 ± 3.7

26.4 ± 4.5

5.8 ± 0.4

   

overall mean

 

10.6 ± 2.5

15.5 ± 3.8

4.3 ± 1.2

3

Muc1 IC2/+ ; Rosa26 EYFP/+

pups (P0)

2 mg TM

SQ

21 d

1

21.7

ND

8.9

    

2

22.8

 

7.8

    

3

51.1

 

13.5

    

mean

31.9 ± 9.6

 

10.1 ± 1.7

  1. Three "pulse-chase" lineage-labeling experiments were performed, as described in the text, in which tamoxifen was administered (by oral gavage to adults, or subcutaneous injection to neonates) and mice sacrificed after chase periods of 7-120 days. For each sample, we counted total cells per field (DAPI), acinar cells (amylase+) and duct cells (cytokeratin-19+), as well as the number of cells double-positive for these markers and for the lineage tracer. From these counts, we derived the labeling efficiency (expressed as a percentage) of total cells (DAPI), acinar (AMY) and duct (CK19) cells. Shown are summary data for every mouse analyzed, as well as mean values +/- standard error. ND, not determined.
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BMC Developmental Biology

ISSN: 1471-213X

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