Figure 4
From: An allele separating skeletal patterning and spermatogonial renewal functions of PLZF
Expression of PLZF and alignment of BTB domains in region of 7t mutation. (A) Western blot analysis of testis proteins from the indicated genotypes with the mouse anti-PLZF antibody. All the protein samples were prepared from testes of adult animals (>3 months) except for the lu/7t and lu/lu samples, which were from 1 month old mice; this was to ensure that the result was not falsely negative due to depletion of spermatogonia (the cells producing PLZF) that occurs over time into adulthood in lu mutants. (B) The 7t mutation region is highly conserved amongst vertebrate Plzf alleles, but not other BTB domains in BTB domain-containing zinc finger proteins. Zfish = Danio rerio; chick = Gallus gallus.