Figure 1
From: An allele separating skeletal patterning and spermatogonial renewal functions of PLZF
Positional cloning of the seven toes ( 7t ) mutation. (A) Shown are the genotyping results for two affected animals (2554 and 3518, both showing polydactyly) harboring critical recombination events. This localized 7t to a 0.65 Mb interval between SSLP markers D9Mit154 and D9Mit99. (B) The intron/exon structure of Plzf on mouse chromosome 9. (C) Sequencing chromatograms of wild type (+/+) and Plzf7t/7tcDNA reveal an A to G change at nt 379 of the Plzf cDNA. (D) Schematic diagram of the PLZF proteins with the indicated BTB and zinc-finger (ovals with "Z") domains. The 7t mutation changes codon 44 from glutamic acid to glycine.