Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

Scholz, Birger; Alm, Henrik; Mattsson, Anna; Nilsson, Anna; Kultima, Kim; Savitski, Mikhail M; Fälth, Maria; Sköld, Karl; Brunström, Björn; Andren, Per E; Dencker, Lennart

doi:10.1186/1471-213X-10-30

BMC Developmental Biology

Table 2 Differentially expressed peptides

From: Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

Precursor	Peptide	Sequence	F-test FDR adj. p-value¹	Age effect log2 fold change	Sex effect log2 fold change	EE2 effect log2 fold change	Interactions²
ACBP	ODN-peptide	TVGDVNTDRPGMLDF	p < 0.001	3.80***			p < 0.01
CBLN1	Cerebellin-1	SGSAKVAFSAIRSTNH	n.s.				n.s.
CBLN4	Cerebellin-4	ANSKVAFSAVRSTN	n.s.				n.s.
CBLN4	Cerebellin-4	SKVAFSAVRSTN	n.s.				n.s.
GRP	GRP	APLQPGGSPALTKIYPR	p < 0.001	1.08***	-0.59***		n.s.
GnIH	GnIH-RP2	ARSSIQSLLNLSQ	p < 0.001	3.41***	-0.94*		p < 0.01
PNOC	PNOC	AVASPLQVSELL	p < 0.001	1.62***			n.s.
PNOC	Nociceptin	YGGFIGVRKSARKWNNQ	p < 0.001	1.56***			p < 0.05
PNOC	Nociceptin	YGGFIGVRKSA	p < 0.01	0.75***		0.45*	p < 0.05
PNOC	Neuropeptide1	GSWPAARGVQ	p < 0.001	1.24***		0.29*	n.s.
PNOC	Neuropeptide2	FSEFLKQYLGMSPR	p < 0.001	1.17***			n.s.
PNOC	Neuropeptide2	SEFLKQYLGMSPR	p < 0.001	2.24***			n.s.
PENK	Precursor	ELYHPESEDEANGGEILA	n.s.				n.s.
PENK	Precursor	SPELEDEAKELQ	p < 0.001	0.92***			n.s.
PENK	Precursor	SPELEDEAKEL	p < 0.001	2.36***	0.57*		n.s.
PENK	Precursor	LEDEAKELQ	p < 0.001	1.76***			n.s.
PENK	Precursor	VGRPEWWLDYQ	p < 0.001	0.96***		0.51*	p < 0.05
PENK	MERF	YGGFMRF	p < 0.001	1.59***			n.s.
PENK	MERSL	YGGFMRSL	p < 0.001	0.95***		0.35*	n.s.
PENKB	Precursor	PKLKWDNQ	p < 0.01	2.36***			n.s.
PPT	CTFP	SLNSGSSERSIAQNYE	n.s.	0.66**			n.s.
PPT	CTFP (1P)	SLNSGSSERSIAQNYE	p < 0.001	1.28***			n.s.
PPT	CTFP (2P)	SLNSGSSERSIAQNYE	p < 0.05	0.92***			n.s.
PPT	SP	RPRPQQFFGLM	p < 0.001	1.80***			n.s.
PPT	NPK	DAGYGQISH	n.s.			-0.99***	p < 0.05
PPT	NKA	HKTDSFVGLM	p < 0.001	1.78***			n.s.
SCG1	SCG1	IHEGEEGEAEEE	n.s.				n.s.
SCG2	Secretoneurin	TNEIVEEQYTPQSL	n.s.	0.40*			n.s.
SCG2	SCG2	SGKLSFLEDE	p < 0.001	2.40***			p < 0.05
SCG5	CTP	SVNPYLQGKRLDNVVA	p < 0.001	1.12***		0.18*	n.s.
SCG5	CTP	SVNPYLQGKRLDNVV	p < 0.001	2.64***			n.s.
SMS	SMS-14	KNFFWKTFTSC	p < 0.001	2.62***			p < 0.05
SMS	SMS-14	FWKTFTSC	p < 0.001	2.70***			n.s.
SMS	SMS-28	SANSNPALAPRE	p < 0.001	1.03***			n.s.
VIP	VIP	AVFTDNYSRF	p < 0.001	2.76***			n.s.
CRMP-2		APPGGRANITSLG	p < 0.01			-0.82**	p < 0.01
PPIA/FKBP12		ANAGPNTNGSQFFICTA	p < 0.001	-0.55***	-0.29*		n.s.
Thymosin beta		SDKPDMAEIEKFDK	n.s.				n.s.

¹. The F-test describes the significance of the linear model that incorporates the three parameters in this study: age (ed12 and ed17, sex (male and female), and exposure (controls and EE2 exposed). There are three levels of statistical significance for the parameter effects: p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). A minus sign (-) in the age effect column means that the peptide is more highly expressed in ed12 than ed17. A minus sign (-) in the sex effect column means that the peptide is more highly expressed in males compared to females. A minus sign (-) in the EE2 effect column means that the peptide is more highly expressed in controls compared to exposed. ². The significance of the interaction effects was calculated by Walds F-test. n.s. means non-significant.

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