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Table 2 Differentially expressed peptides

From: Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

Precursor Peptide Sequence F-test
FDR adj. p-value1
Age effect
log2
fold change
Sex effect log2 fold change EE2 effect
log2 fold change
Interactions2
ACBP ODN-peptide TVGDVNTDRPGMLDF p < 0.001 3.80***    p < 0.01
CBLN1 Cerebellin-1 SGSAKVAFSAIRSTNH n.s.     n.s.
CBLN4 Cerebellin-4 ANSKVAFSAVRSTN n.s.     n.s.
CBLN4 Cerebellin-4 SKVAFSAVRSTN n.s.     n.s.
GRP GRP APLQPGGSPALTKIYPR p < 0.001 1.08*** -0.59***   n.s.
GnIH GnIH-RP2 ARSSIQSLLNLSQ p < 0.001 3.41*** -0.94*   p < 0.01
PNOC PNOC AVASPLQVSELL p < 0.001 1.62***    n.s.
PNOC Nociceptin YGGFIGVRKSARKWNNQ p < 0.001 1.56***    p < 0.05
PNOC Nociceptin YGGFIGVRKSA p < 0.01 0.75***   0.45* p < 0.05
PNOC Neuropeptide1 GSWPAARGVQ p < 0.001 1.24***   0.29* n.s.
PNOC Neuropeptide2 FSEFLKQYLGMSPR p < 0.001 1.17***    n.s.
PNOC Neuropeptide2 SEFLKQYLGMSPR p < 0.001 2.24***    n.s.
PENK Precursor ELYHPESEDEANGGEILA n.s.     n.s.
PENK Precursor SPELEDEAKELQ p < 0.001 0.92***    n.s.
PENK Precursor SPELEDEAKEL p < 0.001 2.36*** 0.57*   n.s.
PENK Precursor LEDEAKELQ p < 0.001 1.76***    n.s.
PENK Precursor VGRPEWWLDYQ p < 0.001 0.96***   0.51* p < 0.05
PENK MERF YGGFMRF p < 0.001 1.59***    n.s.
PENK MERSL YGGFMRSL p < 0.001 0.95***   0.35* n.s.
PENKB Precursor PKLKWDNQ p < 0.01 2.36***    n.s.
PPT CTFP SLNSGSSERSIAQNYE n.s. 0.66**    n.s.
PPT CTFP (1P) SLNSGSSERSIAQNYE p < 0.001 1.28***    n.s.
PPT CTFP (2P) SLNSGSSERSIAQNYE p < 0.05 0.92***    n.s.
PPT SP RPRPQQFFGLM p < 0.001 1.80***    n.s.
PPT NPK DAGYGQISH n.s.    -0.99*** p < 0.05
PPT NKA HKTDSFVGLM p < 0.001 1.78***    n.s.
SCG1 SCG1 IHEGEEGEAEEE n.s.     n.s.
SCG2 Secretoneurin TNEIVEEQYTPQSL n.s. 0.40*    n.s.
SCG2 SCG2 SGKLSFLEDE p < 0.001 2.40***    p < 0.05
SCG5 CTP SVNPYLQGKRLDNVVA p < 0.001 1.12***   0.18* n.s.
SCG5 CTP SVNPYLQGKRLDNVV p < 0.001 2.64***    n.s.
SMS SMS-14 KNFFWKTFTSC p < 0.001 2.62***    p < 0.05
SMS SMS-14 FWKTFTSC p < 0.001 2.70***    n.s.
SMS SMS-28 SANSNPALAPRE p < 0.001 1.03***    n.s.
VIP VIP AVFTDNYSRF p < 0.001 2.76***    n.s.
CRMP-2   APPGGRANITSLG p < 0.01    -0.82** p < 0.01
PPIA/FKBP12   ANAGPNTNGSQFFICTA p < 0.001 -0.55*** -0.29*   n.s.
Thymosin beta   SDKPDMAEIEKFDK n.s.     n.s.
  1. 1. The F-test describes the significance of the linear model that incorporates the three parameters in this study: age (ed12 and ed17, sex (male and female), and exposure (controls and EE2 exposed). There are three levels of statistical significance for the parameter effects: p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). A minus sign (-) in the age effect column means that the peptide is more highly expressed in ed12 than ed17. A minus sign (-) in the sex effect column means that the peptide is more highly expressed in males compared to females. A minus sign (-) in the EE2 effect column means that the peptide is more highly expressed in controls compared to exposed. 2. The significance of the interaction effects was calculated by Walds F-test. n.s. means non-significant.