Figure 6
From: Cell cycle arrest by a gradient of Dpp signaling during Drosophila eye development
Expression and requirement for homothorax. (A) Homothorax (green) is expressed in the anterior eye disc but repressed anterior to the morphogenetic furrow. Levels gradually reduce, starting while cells are still cycling (CycB in magenta); (B) Hth channel from panel A; Blue arrow shows onset of arrest, as measured by loss of Cyc B (C) CycB channel from panel A; (D) Clones of cells expressing GFP (green) and mutant for hth are recovered in posterior eye regions where cell proliferation stops earlier in development, but rarely recovered in the anterior eye. CycB in magenta; (E) Large hth mutant clones are recovered readily in a heterozygous Minute background. Clones identified by absence of beta-galactosidase labeling; (F) Clones of cells expressing both GFP (green) and TkvQD as well as mutant for hth are recovered very poorly in all locations of the eye disc. CycB in magenta.