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Figure 1 | BMC Developmental Biology

Figure 1

From: Functional conservation between rodents and chicken of regulatory sequences driving skeletal muscle gene expression in transgenic chickens

Figure 1

The rat MLC1/3 locus and lentiviral construct. Top: The rat MLC locus consists of two separate promoter elements which generate two alternatively spliced transcripts. A downstream enhancer augments expression from both promoters in skeletal muscle. Stippled exons are specific for MLC3. The internal MLC3 promoter and the downstream enhancer were cloned upstream of a lacZ reporter construct in an EIAV replication defective lentiviral vector to generate pONY-MLZ. Restriction sites for HindIII (red arrows) and Sph1 (black arrows) are indicated. Bottom: Sequence comparison of homologous downstream regions of the MLC1/3 locus of eutherans and chickens. Grey boxes highlight core enhancer elements identified in human, mouse, and rat. The internal basepairs of the E box of Site A are changed to the sequence of site B in the chicken. Site C is in reverse orientation in the chicken to that in mammals.

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BMC Developmental Biology

ISSN: 1471-213X

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