Glycogen Synthase Kinase-3 (GSK3) activity and relative expression during A. aegypti embryo development. The GSK3 activity (open lozenge) and GSK3 transcripts levels (black down-pointing triangle) (normalized by rp49 cDNA) were measured on different hours of embryo development. Each experiment was replicated three times and error bars represent standard deviation of sample means. Insert shows GSK3 transcripts levels in ovaries of A. aegypti females dissected in different hours after blood meal (**p < 0.01; ***p < 0.001, ANOVA). The dashed line indicates the germ band retraction stage 24 HAE.