Figure 4From: BMP-2 functions independently of SHH signaling and triggers cell condensation and apoptosis in regenerating axolotl limbsExpression pattern, by whole mount in situ hybridisation, for BMP-2 (A-C) and SOX-9 (D-F) at the ED stage in regenerating limb treated with cyclopamine from the time of amputation until fixed at ED. Left column (A, D, G) shows control animals (treated with the carrier 0.4 μL EtOH/mL), middle column (B, E, H) shows the animals treated with 1 μg/mL of cyclopamine and right column (C, F, I) shows the animals treated with 2 μg/mL of cyclopamine. All pictures show a dorsal view of the limbs with the posterior side on the left. The bottom row (G, H, I) show the skeletal staining (Victoria blue) at the end of the treatments when the control animals had completely regenerated.Back to article page