Figure 8
In situ hybridization. Expression of mortalin at advanced stages (days 12 - 21) of gut regeneration. (A) Posterior rudiment on day 12 after autotomy. (B) Growing posterior tip of the anterior rudiment on day 12 after evisceration. (C) The wall of the esophageal stump on day 12 after evisceration. (D) The wall of the newly regenerated posterior regions of the esophagus on day 21 after evisceration. (E) The second descending part of the newly regenerated intestine on day 21 after evisceration. The inserts show higher magnification view of the asymmetrical expression of mortalin in the mesothelium of the mesentery attachment and also strongly labeled singly scattered cells in other regions of the mesothelium. de - digestive (luminal) epithelium; ct - connective tissue layer; m - mesothelium. Scale bars = 200 μm in (A) and (E); 500 μm in (B); 25 μm in (C), (D) and (E insets).