Figure 3
From: β-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis
Development of the osteoblast lineage is affected in the sβcatAx2 mice. Immunostaining analysis examines the expression of osteogenic markers, Runx2 (A, B), Osterix (C, D), Col1 (E, F) and Osteopontin (G, H), in the one week old control (A, C, E, G) and sβcatAx2 (B, D, F, H) mice. Brackets indicate the expression domains. (I) The expression of sβ-cat accelerates osteoblast differentiation in vitro. The control (Axin2rtTA; β-cateninΔEx3Fx/+) and mutant (Axin2rtTA+; TRE-Cre+; β-cateninΔEx3Fx/+) calvarial cells, isolated from the newborn skulls, were induced for differentiation for 6 days. Alkaline phosphatase liquid assay shows that the presence of Dox has minimal effect on osteoblast differentiation in the controls. However, the inducible expression of sβ-cat results in accelerated differentiation. The diagram is a representative of two independent experiments. Scale bars, 50 μm (A-H).