Multiple phenotypic changes in mice after knockout of the B3gnt5gene, encoding Lc3 synthase—a key enzyme in lacto-neolacto ganglioside synthesis

Table 2 Genome typing distribution in three groups of Lc3 synthase knockout mice*

Group	Number of mice	Wt/Wt (% of total)	Wt/KO (% of total)	KO/KO (% of total)
Fetus^†
Conventional	28	7 (25)	12 (43)	9 (32)
Cre-loxP	14	2(14)	8 (57)	4 (29)
Adult^§
Conventional	127	40 (32)	66 (52)	21 (16)**
Cre-loxP	251	126 (50)^§§	97 (39)^‡‡	28 (11)^§§
Dead^§
Conventional	14	0**	4 (29)^‡‡	10 (71)^§§

Abbreviations: KO, knockout; Wt, wild type.
*Genome typing was done at day E17 for fetuses, after weaning for adult mice, and immediately after death in postnatal days 15-40.
^†Fetuses were obtained through dissection of a dam as described in Materials and Methods.
^§Values and percentages in the Adult and Dead groups (Wt/Wt, Wt/KO, and KO/KO) were significantly different from the theoretical number based on Mendel's law.
**P < 0.05.
^§§P < 0.00001 by binomial test.
^‡‡P < 0.0004.

ISSN: 1471-213X