Multiple phenotypic changes in mice after knockout of the B3gnt5gene, encoding Lc3 synthase—a key enzyme in lacto-neolacto ganglioside synthesis

Table 1 Ganglioside concentration of Lc3 synthase knockout mice*

Tissue and Genome Type^†	Sialic Acid (μmol/g tissue)	GM1 %SA	GD1a %SA	GD1b %SA	GT1b %SA	3'-LM1 (nmol/g tissue)	3'-isoLM1 (nmol/g tissue)
Adult Cerebellum
KO/KO	2.64 ± 0.33	7.45 ± 1.50	8.78 ± 1.23	18.9 0 ± 1.17	47.6 ± 1.69	ND	ND
Wt/KO	2.70 ± 0.28	7.00 ± 1.90	7.63 ± 0.75	18.58 ± 1.71	51.8 ± 3.14	0.14 ± 0.12	ND
Wt/Wt	2.64 ± 0.25	6.15 ± 0.85	7.68 ± 0.81	19.13 ± 1.74	51.3 ± 2.46	0.24 ± 0.13	ND
Adult Cortex
KO/KO	3.85 ± 0.38	8.35 ± 1.06	33.7 ± 4.14	8.90 ± 0.49	38.7 ± 1.83	ND	ND
Wt/KO	3.95 ± 0.66	8.38 ± 1.42	37.3 ± 4.35	8.58 ± 1.04	38.0 ± 3.08	ND	ND
Wt/Wt	3.73 ± 0.37	7.35 ± 0.55	34.7 ± 3.71	8.90 ± 0.42	40.2 ± 2.04	0.18^§	ND
Fetus brain
KO/KO						ND	ND
Wt/KO						1.40 ± 0.00	ND
Wt/Wt						5.00 ± 0.42	ND

KO, knockout; ND, not detected; Wt, wild type; %SA, percentage of sialic acid.
*All values are means ± SD. The lacto-series ganglioside assay was based on lipid extracts corresponding to 5 mg of tissue that were assayed by TLC-immunostaining. Detection limits were as follows: 3'-LM1, 0.04 nmol/g tissue; 3'-isoLM1, 0.04 nmol/g tissue.
^†Four samples were in each group for the cerebellum assay and the cortex assay. Two samples were in each group from different parental pairs for the fetal brain assay.
^#Remaining gangliosides constituting 100% of total sialic acid: GM3, GM2, GD3, GD2, and GQ1.
^§Data from one mouse detected.

ISSN: 1471-213X