Figure 3

Laminin alpha 5 null hair follicles normally express dermal papilla markers. A: Images of indirect immunofluoresence for mouse LM511 (green) in E16.5 wild type and lamα5-/- mice (as indicated) showing a lack of antigen in null animals A': The same images as (A) indicating the Stage 2 hair follicles present in each view. B: Quantitation of p75-positive cells in dermal condensate of Stage 1 and Stage 2 hair follicles of E16.5 wild type and lama5-/- mice. C-E: Confocal images of indirect immunofluoresence for dermal markers CD133 (C), p75 (D) and syndecan-1 (E) in wild type and lamα5-/- Stage 1 and Stage 2 follicles within E16.5 skin. Hoechst (blue) labels cell nuclei. Dashed lines indicate epidermal/dermal boundary of hair follicles. Scale bars represent 10 μm. Abbreviations: DP, dermal papilla; HF, hair follicle.