Figure 4

The functionality to search fish and insertions. (A) "Find image" is a main page to find gene trap and enhancer trap fish with desired patterns. Thumbnails of images of fish lines appear when an expression pattern and/or construct name is selected in the "by region", "by construct" and "by number" columns on the left. Here, ALL in "by region", SAG in "by construct" and ALL in "by number" are chosen, and all "SAG" transgenic fish are seen on the right. These can be seen also as a list style by clicking "Show Image List" or "Show Line List". Icons next to the line name link to the fish status data, the insertion data and the z! and e! genome browsers.

(B) "Find UAS" allows users to find transgenic fish carrying a reporter or an effector gene downstream of the Gal4 recognition sequence. Here, double transgenic fish carrying the hspGGFF1B insertion and UASGFP or UASRFP are shown. Icons next to the line names link to the fish status data, the insertion data and the z! and e! genome browsers.

(C) "Find insertion" allows users to search the insertion data by filling in the blanks. Here, "HG" is typed in "insertion name" and the "exon" checkbox is checked, and all insertions that are created by using the T2KHG construct and located within an exon are seen.

(D) "Gene-to-Insertion Search" allows users to find insertions located near a gene of interest. Here, "fox" is typed, and all insertions located within a distance of 100-kb from the fox genes are picked up.