The mec-3 promoter did not enhance the titration effect. The sequence from the BbsI site to the mec-3 translation start site contained the mec-3 promoter. Plasmid pJC11, which contained the mec-3 promoter, and plasmid pJC10, which did not contain the mec-3 promoter, were transformed into strain TU2562. Three lines were generated from each plasmid. The bar graphs show the positions of ALMs from 100 animals for each of the three lines. The percent of misplaced ALMs and the number of ALMs scored are shown on the right.