Figure 4

ALM positions in strains transformed with mec-3 upstream regions. Data from strain TU2562 is included to show the normal distribution of ALMs. The diagrams on the left indicate the regions of mec-3 in each plasmid. ATG marks the translational start site. Bsa BI, BglII, PstI and BbsI are restriction enzyme cutting sites. Filled rectangles represent mec-3 exons. The open rectangles marked GFP represent green fluorescent protein encoding sequences. pJC8 begins 2372 bp upstream of the mec-3 translational start site and is fused to GFP at the end of exon 3. The bar graphs show the number of ALMs at each position. The number of ALMs is on the Y-axes and the position is on the X-axes. For most constructs, 100 animals from each of three separate transgenic lines were scored for ALM positions. For two strains transformed with pJC3, 200 animals were scored. The percent refers to the percent of ALMs anterior to position 8, and n is the total number of ALMs scored. The system for scoring ALM positions is described in Figure 1.