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Table 1 Nuclear swelling and polar body formation at 15 h after fusion in porcine embryos reconstructed by transferring confluent (CON) or nocodazole-treated (NOC) cells into enucleated M I oocytes

From: Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium

      

No. of embryos with

       

Condensed chromatin

   
  

No. of

  

No

  

One &

 

Swollen nucleus

 

No. of

complexes

No. of embryos

chromatin

  

polar

Total

  

Total

Donor

trials

Treated

Fused (%)a

Examined

Surviving

/nucleus

One

Two

body (%)b

(%)b

One

Two

(%)b

CON

3

128

77 (60.2)c

40

40

4

34

0

0(0)

34 (85.0)c

2

0

2 (5.0)

NOC

4

248

64 (25.8)d

32

30

10

9

6

4(13.3)

19 (63.3)d

0

1

1 (3.3)

  1. aPercentage of complexes treated. bPercentage of embryos surviving. c-dValues with different superscripts are significantly different (P < 0.05).
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BMC Developmental Biology

ISSN: 1471-213X

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