Fig. 3From: Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigasGene expression of CgNOS in Pacific oysters at different larval stages, larvae exposed to different metamorphosis inducers and spat 24 h after metamorphosis induction as well as metamorphosis percentages (%) in Pacific oyster larvae after exposure to inducers. A & C) C. gigas larvae, 18 dpf (d) in January 2018 experiments, exposed to epinephrine (EPI) and MK-801 (MK) at 10− 4 M, L-DOPA at 10− 5 M and ifenprodil (IP) at 10− 6 M and sampled at 4 hpe and 6 hpe. B & D) C. gigas larvae, 17 dpf or 18 dpf for ODQ in March 2019 experiments, exposed to EPI, MK-801, SMIS and 7-NI at 10− 4 M, AGH at 10− 3 M and to ODQ at 5 × 10− 5 M and sampled 3 hpe and 6 hpe. S: spat, C: control spat. Error bars represent standard error. Different lower-case letters represent significant differences with p < 0.05; *: significance not calculated due to differences in sampling daysBack to article page