Fig. 8
Evaluation of the specificity of dmrt2a-MO. a Alignment between wildtype dmrt2a sequence, dmrt2aΔ100 sequence and dmrt2a-MO. The MO is represented in the sense orientation. In bold caps is represented the start codon, in green the indels and in orange the 3’end of the 5’UTR. b, c Validation of the specificity of dmrt2a-MO using (b) myod1 (14-somite stage) and (c) spaw (20-somite stage). The embryos shown illustrate the different phenotypes obtained in myod1 expression. d, d’ Evaluation of p53 levels by qPCR in (d) Region 1 (somites) and (d’) Region 2 (tailbud/LRO) after injecting dmrt2a-MO in wildtype embryos. UTR: Untranslated Region, L: left, R: right, B: bilateral, NAE: no anterior expansion, Sym: symmetric, Asym: asymmetric, ctrMO: control morpholino, ns: not significant. P-values were generated using a two-tailed t-test