Fig. 7

Survival of the tbx5 morphants and IGF-I-treated tbx5 morphants. The embryos were examined longitudinally under a microscope for up to 96 hpf. The survival rate of the WT group at 96 hpf was approximately 90%. The MIS and WTIGF1 groups had similar survival rates as the WT group. The MO group had the lowest survival rate, and the survival rate of the MOIGF1 group was significantly improved. WT: wild-type embryos; MO: tbx5 morphants; MIS: mismatch tbx5 morpholino-treated wild-type embryos; WTIGF1: IGF-I-treated wild-type embryos; MOIGF1: IGF-I- treated tbx5 morphants; hpf: hours post-fertilization. The number of specimens was 50, and the number of independent experiments was 3 in each group