Fig. 2

Expression of Nrp1 and Nrp2 in cranial neural crest cells. a-b) qRT-PCR for Nrp1 (a) and Nrp2 (b) in FACS isolated r1-r2 and r4 NCC streams. a Nrp1 expression normalised to β-Actin in pooled data from four experiments is increased almost 2.5 fold (N = 4; p < 0.05). b Nrp2 expression normalised to β-Actin in pooled data from four experiments is marginally increased in r1-r2-derived NCCs. c-h In situ hybridisation of Nrp1 and Nrp2 from E9.25-E10.0 demonstrates the complexity of Neuropilin expression. Nrp1 is restricted to r4-derived NCCs at E9.25 (c, open arrow head). By E9.5 Nrp1 remained highly expressed within r4-derived NCCs and at lower levels within r1-r2-derived NCCs (d, closed arrow head). e At E10.0 Nrp1 is expressed in r4-derived facioacoustic ganglia (VII/VIII) and a subset of cells within the trigeminal ganglia (V). f Nrp2 expression is restricted to r1-r2-derived NCCs at E9.25 (closed arrow head). g At E9.5 Nrp2 expression is observed in both the r1-r2 and r4 migratory streams (open and closed arrow heads). h By E10.0 Nrp2 is expressed in the developing trigeminal ganglia and within r4 NCC derivatives. e, eye. Scale Bars = 500um