Fig. 3
From: Late stage definitive endodermal differentiation can be defined by Daf1 expression
Daf1-DE is the progenitor of Daf1+ DE. a A schematic drawing showing experimental design. b Quantifications of Foxa2+ and Sox17+ cells by immunocytochemistry after 90 min cell-matrix adhesion assay. c, d The proportion of cells expressing Sox17 was higher in the Daf1 + DE as detected by immunocytochemical analysis (c), and the level of Sox17 expression was higher in the Daf1 + DE as detected by western blot (d), of the sorted DE cells (without plating). e Flow cytometry analysis of the descendent cells of Daf1-DE and Daf1 + DE. Left panels: Daf1, Cxcr4 expression profiles of the sorted Daf1+/- DE cells. Right panels: both Daf1-DE and Daf1 + DE acquired Daf1 expression after 24 h. f The scheme of Fig. 3. Sox17low Daf1-DE differentiated into Sox17high Daf1 + DE. Student’s two tailed t-test. Scale bar; 100 μm