Fig. 6
Analysis of sox8 and twist expression in ALK6 morphant embryos. Embryos were injected with 1.6 pmol morpholino targeted animally in one dorsal blastomere at the 4-cell stage. A lacZ plasmid was co-injected to identify the injected side, embryos were cultured till stage 18 or 24 and stained for LacZ. The neural crest was visualized by in situ hybridization against sox8 at stage 18 and against twist at stage 24. Differences between indicated groups were analyzed using the Χ 2 test. Statistically significant deviations (p-value < 0.01) are indicated by double asterisks; n.s.: not significant. a Representative images of embryos hybridized against sox8 and injected as indicated are shown. The ALK6 MO phenotype was rescued by co-injection of 50 pg alk6 RNA. Asterisks indicate the injected side. The results from three independent experiments are summarized in the graph (b). c Representative images of the uninjected and injected sides of embryos of stage 24 embryos hybridized against twist are shown. Embryos were injected with 1.6 pmol of ALK6 MO 1; for rescue experiments 25 pg of alk3 plasmid DNA or alk6 plasmid DNA were co-injected. The results from three independent experiments are summarized in the graph (d)