Fig. 8
From: A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice
Venus is expressed in the mesoderm and endoderm on the surface of the embryo, but not in the midline. a Alternative views (ventral, frontal, posterior) depicting regions of Venus expression (green) in Gata6 H2B-Venus/+ embryos at E7.75. Venus is excluded from the midline. Surface views are renderings of z-series images. Anterior (A), posterior (P), right (R), left (L), proximal (Pr), distal (D). Nuclei are stained with Hoechst (blue). b Immunostaining for BRACHYURY (red) labels the midline in Gata6 H2B-Venus/+ embryos at E7.75. Transverse sections showing mesoderm (meso) and endoderm (endo) layers. c Immunostaining for FOXA2 (red) shows very high expression in the midline and lower levels of expression in the surface endoderm in Gata6 H2B-Venus/+ embryos at E7.75. Venus (green) coincides with FOXA2 in most cells of the surface endoderm, however it is not co-expressed in the midline. Transverse sections showing mesoderm (meso) and endoderm (endo) layers