Fig. 5
From: Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species
The highly conserved SPCI contains recognised TF binding motifs. a A detailed plot of conservation in the SPCI, covering bases from −402 to −296 in the 5’ flank of mouse Sry. Conservation is estimated by RPhast from a multiple alignment generated by MUSCLE using repeat-masked flanking sequences of 17 species. This plot is aligned with (b) the sequence of the multiple alignment from which the conservation probabilities were derived. The human TSS is indicated by a small green box (TSS in other species is largely uncharacterised). A red underline indicates a putative GATA4 binding site present in the mouse whose function is supported by in vivo evidence. c A representative selection of logos for motifs with binding sites predicted by FIMO within the sequence. Details for motifs, and p-values calculated by FIMO are, from left to right: Oct/Sox (JASPAR MA0142.1, p-value < 10−4), Meis (Jolma MEIS1_DBD, p-value < 10−4), Sox (JASPAR MA0078.1, p-value < 10−4), Sp (JASPAR MA0079.1, p-value < 10−5), WT1 (Jolma EGR1_DBD, p-value < 10−4), Sp (JASPAR MA0079.2, p-value < 10−4), Forkhead box (Jolma FOXD2_DBD, p-value < 10−5), Oct/Sox (JASPAR MA0143.1, p-value < 10−4)