Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs including brain, pancreas, liver, kidney, lung, and intestine

Table 1 Optimized ETC. conditions for various tissues

Organ	Optimized ETC. time (days)	Constant current (mA)	Varying voltage (V)	Replacement interval of clearing solution (days)
Brain	Whole brain: 12-16	280	22 ~ 26	5
Brain	3-mm-thick-block: 3	280		N/A
Pancreas	8-12	250		7
Kidney	18-22	280		7
Liver	18-22	280		7
Intestine	8-12	250		7
Lung	13-17	250		7

n = 30 for optimization of each organ clearing. ETC., electrophoretic tissue clearing; mA, milliampere; V, voltage; N/A, not available.

ISSN: 1471-213X