Figure 1
From: 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B
The expression of 14-3-3ε in fertilized mouse eggs. A: the mRNA expression levels of 14-3-3 isoforms at G1 phase of mouse fertilized eggs. mRNA of 150 fertilized mouse eggs was extracted in G1 phase. RT-PCR products using primers for 14-3-3 isoforms are observed in ethidium bromide-stained agarose gel. 14-3-3ε, 14-3-3β, 14-3-3γ, 14-3-3ζ, 14-3-3η, 14-3-3σ, and 14-3-3τ represent seven 14-3-3 isoforms. B: the mRNA levels of 14-3-3ε at G1, S, G2 and M phases (P >0.05). mRNA of 150 fertilized mouse eggs was extracted in G1, S, G2 and M phases, respectively. RT-PCR products using primers for 14-3-3ε (800 bp) and β–actin (300 bp) are observed in ethidium bromide-stained agarose gel (upper panel). Densitometric quanitification represents the mRNA levels of 14-3-3ε (lower panel) (P >0.05). C: Western blot analysis of 14-3-3ε protein expression (P >0.05). Immunoblots were performed for expression of 14-3-3ε (29 Mr × 10−3) and β-actin (43 Mr × 10−3) using anti-14-3-3ε or anti-β-actin antibodies (upper panel). Densitometric quanitification represents the protein expression of 14-3-3ε (lower panel) (P >0.05). 300 fertilized eggs are loaded onto each lane. Molecular weight of proteins (Mr × 10−3) is indicated. A x2 test was used to evaluate the differences of endogenous 14-3-3ε mRNA expression levels or protein expression of 14-3-3ε between multiple experimental groups. Bars represent means ± S.D of three independent experiments.