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Table 1 FACS analysis of control and ERG shRNA treated embryoid bodies.

From: ERG is required for the differentiation of embryonic stem cells along the endothelial lineage

 

Day 6

Day 8

Day 10

 

Control

ERG ShRNA

Control

ERG ShRNA

Control

ERG ShRNA

CD 41

1.4%

1.9%

2.5%

2.1%

2.3%

5.5%

CD 45

0.8%

1.3%

0.7%

0.5%

2.4%

3.1%

VE-Cadherin

5.8%

1.5%

19.3%

10.4%

26.8%

5.7%

Endoglin

23.6%

1.8%

26.0%

19.3%

25.7%

10.2%

Tie2

10.5%

9.3%

19.6%

17.4%

20.6%

13.2%

VEGF-R1

26.6%

25.9%

34.9%

25.4%

21.8%

16.4%

  1. Flow cytometric analysis of control and ERG shRNA treated EBs at different time points (day 6, 8, 10) using antibodies directed against endothelial (VE-cadherin, endoglin, Tie2, and VEGF-R1) and hematopoietic (CD41, CD45) cell surface markers.