Figure 4

Evaluation of ERG expression in the developing heart. Sagittal sections of E9.5 mouse embryos with DAPI staining (left upper panel). High power magnification of the developing cardiac ventricular chamber (upper right) and common atrial chamber. The ventricular chamber is highly trabeculated and expression of the endocardial surface is shown by VE-cadherin staining (red). ERG (green) co-localizes with VE-cadherin expressing cells in both the atrium and ventricular chambers. The images were obtained with Leica fluorescence microscope at 10× and 40× magnification. Scale bars represent 80 μm and 70 μm (top and bottom panel, respectively).