Figure 2

Co-localization of Prox1 protein with different cell type markers in the lateral line system. Immunofluorescence showing Prox1 (red) in different GFP transgenic lines (green) at 48, 72 and 96 hpf: (A) SqET20 that labels mantle cells; (B) cldnb::GFP that labels all cells that form the neuromast; (C) SCM1 that labels presumptive progenitor cells; (D, E) pou4f3::GFP that labels hair cells. (F) Schematic representation of a neuromast showing the different cell types. Prox1 expression is represented as filled cells and can be seen among hair cells (red) and underlying supporting cells (green) and/or progenitor cells (yellow). Prox1 is absent from mantle cells (blue). Scale bar = 5 micron.