Figure 8
Live imaging cell fate during mouse cardiac morphogenesis. Panels show 2D (bright field) and 3D (green and red channels) laser scanning confocal images of photoconversion cells on the right side (ROI) of the cardiac crescent (cc) of an E7.5 CAG::KikGRTg/+ embryo (A-E). After 12 hrs of roller culture the embryo has formed a linear heart tube (lh) and photoconverted cells can be detected on the right side of the linear heart tube (F-J). Widefield image of an E7.5 embryo. Red dashed box shows region of cc depicted in panels A-E (A'). Bright field image of cardiac crescent before culture (A) and embryo after culture (F). Red and green channel (B, E, G and J). Green channel (C and H). Red channel (D and I). White dashed box in panel G shows region depicted in panels H-J.