Figure 6

Wound healing in mosaic mouse corneal epithelium restores the striping pattern. A-C: Fluorescein staining of wound healing of XLacZ^+/- corneal epithelium in ex-vivo culture showing complete wound closure within 24 h. The central green (fluorescein-stained) region in A and B shows the wound area (the white ring superimposed on the cornea in A-C is a reflection artefact that occurred during photography). D: X-gal staining of a healed XLacZ^+/- mosaic eye 24 h after wounding. E-H: Images from time-lapse confocal microscopy of healing of a 1 mm diameter central wound in a PAX6-GFP mosaic corneal epithelium (the white areas are GFP-positive) showing that the wound has closed by 18.75 h. after wounding. I-L: Healing of a peripheral wound showing cell migration and formation of a second whorling pattern. By 18.75 hours this wound has not closed completely.