The DEAD-box protein MEL-46 is required in the germ line of the nematode Caenorhabditis elegans

Table 1 Temperature dependent sterility of mel-46(yt5) hermaphrodites

Genotype mother	Temperature	n	WT (%)^c	Mel (%)^d	Sterile (%)^e	Sterile among mutant (%)^f
mel-46(yt5)/+	20°C^a	240	177 (73.8)	63 (26.3)	0	0
mel-46(yt5)/+	25°C^a	622	460 (74)	110 (17.7)	52 (8.4)	32.10
mel-46(yt5)/mel-46(yt5)	25°C^b	1299	0	674 (51.9)	625 (48.1)	48.33

^a The mothers and the analyzed progeny were permanently kept at the indicated temperature. ^b the analyzed worms where shifted to the temperature indicated as L1 larvae. ^c Phenotypically wild type, these worms were considered to be mel-46(yt5)/+ or +/+.^d Maternal effect lethal, these worms produced embryos, most (at 20°C) or all (at 25°C) of which arrested with less than 100 cells (see Fig.1). ^e These worms did not produce any embryos. ^f Mel and Sterile worms were considered to be mutant.

ISSN: 1471-213X