Figure 2
From: Maternal Oct-4 is a potential key regulator of the developmental competence of mouse oocytes
Stella and Oct-4 proteins are not detected in antral NSN and MIINSN oocytes. (A-C) An SN antral oocyte nucleus which shows the binding of the antibody against Stella (A) around its nucleolus (n); (B) the characteristic ring of DAPI-positive chromatin surrounding the nucleolus. Bar, 3 μm. (D-F) An NSN antral oocyte nucleus negative to the antibody against Stella (D); (E) the nucleus is stained with DAPI. (G-I) A MIISN oocyte that shows the binding of the antibody against Stella dispersed within the whole cytoplasm (G). Bar, 20 μm. (J-L) A MIINSN oocyte negative to the antibody against Stella (J); (H, K) the arrow indicates the MII-plate, the arrowhead indicates the first polar body. (M-O) An SN antral oocyte nucleus that shows the binding of the antibody against Oct-4 localised mainly around the nucleolus. Bar, 3 μm. (P-R) An NSN antral oocyte nucleus negative to the antibody against Oct-4 (P). (S-U) A MIISN oocyte that shows the binding of the antibody against Oct-4 localised around the MII-plate; (U) an enlargement of the insert shown in T. The arrow indicates the MII-plate, the arrowhead indicates the first polar body. Bar, 20 μm. (V-X) A MIINSN oocyte negative to the antibody against Oct-4; (X) an enlargement of the insert shown in W.