Figure 3
From: Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells
A homogenous population of nestin+ neural progenitors derived from a light EB. The light EB derived from TE03 cell line was plated on PDL/laminin and cultured in the NDM for 3 days. To estimate the percentage of differentiated cells expressing nestin or TuJ1, the number of labeled cells was counted from a double-immunolabeled culture at 3 days postplating for nestin (C) and TuJ1 (D), and normalized with the total number of cells determined by counting DAPI stained cells (B) overlapping phase-dark cells (A). This hESC-derived cell population consists of almost all nestin+ progenitors. TuJ1+ neurons (D, pointed by arrows) are barely seen. Scale bar = 100 μm.