Figure 4From: Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprintsExpression of maternal and zygotic Dnmt1s during preimplantation development. A. Time course of Dnmt1s expression at defined preimplantation cleavage stages determined by immunostaining with the UPT82 antiserum. Mo = morula. Bl = blastocyst. B. Time course of Dnmt1s expression during preimplantation development in embryos derived from crosses between wild-type (+/+) female mice and homozygous Dnmt1V/V(V/V) male mice. Inset is bright-field image of immunostained 1-cell embryo. C. Time courses of Dnmt1s expression during preimplantation development in embryos derived from crosses between heterozygous Dnmt1V/+ (V/+) female mice and homozygous Dnmt1V/V(V/V) male mice. The 4-cell and 8-cell embryos in the top row show primarily nuclear Dnmt1s staining whereas 4-cell and 8-cell embryos in the botton row show little or no nuclear Dnmt1s. All seven 2-cell, three out of seven 4-cell and six out of 13 8-cell embryos showed nuclear staining. Bar, 20 μm.Back to article page