Figure 6

Only the AaCHS1a splice variant is expressed when serosa is formed. The relative quantitation of AaCHS1a and AaCHS1b expression was analyzed by qPCR. AaCHS1a has a slight expression at 6-HAE. At 9-HAE on its levels increase significantly, suggesting an AaCHS1a role in synthesis of chitin to the Serosal Cuticle. AaCHS1b is absent until 12-HAE. Both isoforms are expressed in late embryogenesis (31 and 52 hours). Bars represent the mean relative abundance +/- the range based on the SEM (Standard Error of the Mean).