Figure 9

Outgrowth of cranial neural crest cells from stage 10–12 chicken neural tube explants onto ephrin-B1 or EphB2 stripe assay substrates. Representative results from the three experimental conditions. The position of the first set of lanes is revealed by an FITC marker (faint green stripes). In the last image on each row (c, f, j), cells have been double labeled with HNK-1 antibody (red) and DAPI nuclear stain (blue). (a-c) Clustered Fc + FN vs. FN. Cell outgrowth shows no bias for either set of lanes, regardless of Fc protein concentration. (d – f) Clustered ephrin-B1/Fc + FN vs. FN and (g – j) clustered EphB2/Fc + FN vs. FN. Cell outgrowth is even at low stripe protein concentrations, but becomes more restricted to the lanes between the FITC marked stripes with increasing concentrations in the coating solution.