Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans

Table 3 Life span extension by BD is additive with known chemosensory pathways.

Cohort	n	Mean Survival	Median Survival	% extension of Median	p-value
N2 CF	164	24.2 ± 0.4	22.5	-	-
N2 BD	153	32.2 ± 0.7	34.0	51	1.7 × 10^-17
che-3(p801) CF	147	34.3 ± 0.8	34.0	51	2.0 × 10^-21
che-3(p801) BD	168	44.2 ± 1.3	44.0	96	2.6 × 10^-28
N2 CF	70	22.1 ± 0.6	21.0	-	-
N2 BD	112	34.3 ± 0.7	35.0	67	6.0 × 10^-22
che-11(e1810) CF	45	34.7 ± 1.3	35.0	67	8.6 × 10^-15
che-11(e1810) BD	44	45.9 ± 2.1	44.0	110	2.3 × 10^-16
N2 CF	117	24.3 ± 0.5	24.0	-	-
N2 BD	74	36.5 ± 0.8	37.0	54	3.7 × 10^-25
daf-10 (e1387) CF	76	32.7 ± 1.3	34.0	42	4.1 × 10^-07
daf-10 (e1387) BD	56	43.1 ± 2.0	40.0	67	1.7 × 10^-17
N2 CF	119	22.9 ± 0.4	22.0	-	-
N2 BD	131	32.1 ± 0.8	34.0	55	1.1 × 10^-16
osm-3(p802) CF	94	32.4 ± 0.9	32.0	45	5.9 × 10^-18
osm-3(p802) BD	64	42.5 ± 1.8	43.0	95	4.6 × 10^-18
N2 CF	117	24.3 ± 0.5	24.0	-	-
N2 BD	74	36.5 ± 0.8	37.0	54	3.7 × 10^-23
tax-4(p678) CF	113	36.9 ± 0.7	37.0	54	1.9 × 10^-26
tax-4(p678)) BD	155	53.6 ± 0.9	57.0	138	6.0 × 10^-41

Life span was measured for a subset of chemosensory mutants, compared to N2, on plates with food (control fed, CF) or on plates without food (bacterial deprivation, BD). The number of animals (n), mean survival (± standard error, SE), median survival, percent extension of the median, and p-values are given for each strain and condition. Data shown here are pooled from a minimum of two independent trials. P-values were calculated by the Wilcoxon Rank-Sum test using the MATLAB 'ranksum' function.

ISSN: 1471-213X