Figure 4
From: Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells
Differentiation of ES cells with various Rex1 genotypes. A. Photomicrographs of colonies at 4 days cultured with LIF (left) or without LIF (middle), or EBs at 5 days (right) derived from representative ES cells with indicated genotypes for Rex1. Scale bar is 100 μm. B. QPCR analyses of the endoderm marker genes in EBs derived from ES cells with various Rex1 genotypes. Three independent clones with each genotypes were cultured for formation of EBs for 5 days, analyzed separately with normalization by the amount of Gapdh, and plotted with standard deviation against the expression level in wild-type ES cells-derived EBs, set as 1.0. C. QPCR analyses of the stem cell marker genes in ES cells and EBs with various Rex1 genotypes. Three independent clones with each genotypes were cultured for 4 days with LIF or for formation of EBs for 5 days, analyzed separately with normalization by the amount of Gapdh, and plotted with standard deviation against the expression level in wild-type ES cells, set as 1.0.