Figure 2

Expression of loco in the male gonads. A: A UAS-lacZ reporter line revealed that loco was strongly expressed in the adult testes (T) and seminal vesicles (white arrows). B: The β-galactosidase reporter revealed that loco was expressed in the male gonad (black arrow) and in the surrounding fat body tissue. C: The primers shown in Figure 1A were used to establish that loco c1, loco c2, loco c3 and loco c5 were expressed in OrR testes. The band marked with an asterisk is the true PCR product of the 3' end of loco c2 (3'c2) as determined by sequence analysis. D: Several G-protein transcripts were detected in OrR testis. The asterisk indicates the true PCR product of expressed Gα73B as determined by sequence analysis. R: RNA, G: genomic DNA.