Table 1 Primers and PCR conditions
From: R-spondin1 and FOXL2act into two distinct cellular types during goat ovarian differentiation
Experiment | Primer names | Primer sequences | Temp./MgCl2 |
|---|---|---|---|
RT-PCR | Spondin-1 | 5'-CTGCGAGGCCTGCTTCAGCC-3' | 58°C/1,5 mM |
Spondin-3 | 5'-GGAGCAAGGCCCCCACAGAG-3' | ||
RSPO2-1F | 5'-ATGGATTACAGCCASTGCCAAG-3' | 58°C/2,5 mM | |
RSPO2-2R | 5'-TGCCGTGTTCTGGTTTCCAGAC-3' | ||
RSPO3-1F | 5'-AATACATYGGCAGCCAAAACGCC-3' | 59°C/2,0 mM | |
RSPO3-2R | 5'-TGTCAAGGCACTTTCCAAGGTG-3' | ||
RSPO4-3F | 5'-TATCCGCCAGTACGGCAAGTG-3' | 57°C/2,5 mM | |
RSPO4-4R | 5'-CCCTTGTACAGGTAAAACCGCC-3' | ||
FOXL2-9 | 5'-GGCCCCCTGAGCCAGCGCCC-3' | 58°C/1,5 mM | |
FOXL2-10 | 5'-CCCCGACGCTGAGGTGCCCG-3' | ||
GAPDHs | 5'-AGGCCATCACCATCTTCCAG-3' | 58°C/1,5 mM | |
GAPDHas | 5'-GGCGTTGGACAGTGGTCATAA-3' | ||
5'RACE | Spondin-2 | 5'-CCCTCCGGAAGCCACAGAGC-3' | 58°C/1,5 mM |
Spondin-5 | 5'-GCCTTTGGCACAGGCCTGGC-3' | ||
Spondin-6 | 5'-AGGATGGCAAGCAGACGCCC-3' | ||
pSG5 constructs | Spondin-ATG1bis | 5'-CCGGCGAGTGACTATGCGGC-3' | 60°C/1,5 mM |
Spondin-ATG2 | 5'-GTGTGGTGGCCTTGGTTCTG-3' | ||
Spondin-TGA | 5'-TCGCATGGACCGGGAGGCTG-3' | ||
In situ hybridization | Spondin-12 | 5'-CCATCTGGGAGAGGTAGAATC-3' | 55°C/1,5mM |
Spondin-13 | 5'-CACCTCCTCTGAAAAACTTCCC-3' | ||
HIS-RSPO1-1F | 5'-GGCGCTCGAAGACGCAAGGG-3' | 58°C/1,5 mM | |
HIS-RSPO1-2R | 5'-GGATAAAGTCACACAGCTGG-3' |