Figure 6

RSPO1, FOXL2, c-Kit, WNT4, β-catenin immuno-detection and RSPO1 in situ hybridization on goat ovaries. a) Double immuno-detection of RSPO1 (green) and c-Kit (red) on 40 dpc ovaries, showing a co-localization of both proteins on germ cell membranes. The right pictures correspond to a 5.0 enlargement of the red rectangles depicted on the medium picture. Scale bars: 50 μm. b) In situ hybridization of RSPO1 specific probes on 45 dpc ovaries. The medium picture correspond to a 5.0 enlargement of the red rectangles depicted on the left picture, showing a positive germ cell. Note the great majority of cortical cells (somatic + germinal) expressed RSPO1. Scale bars: 25 μm. c) Immuno-detection of RSPO1, WNT4 and b-catenin on 50 dpc ovaries. Note the similar localization of these 3 proteins, mainly on germ cells. m = mesonephros; co = cortex; Scale bars: 100 μm.